Products

Helicobacter pylori Real time PCR Assay

Helicobacter pylori is a gram-negative bacterium formerly known as Campylobacter pylori. The bacterium is helical-shaped and flagellated. Bacterial infection can cause peptic ulcers in 10 to 20% of people, lymphoma of the gastric mucosa-associated lymphoid tissue, and a risk of 1 to 2% acquiring gastric adenocarcinoma stomach cancer. H. pylori spreads through direct contact with saliva, vomit, or stool from person to person. Contaminated water can also transmit H. pylori, particularly in areas with poor sanitation and clean water.

Potential Research Use (RUO) Applications:

H. pylori is diagnosed through various methods, including a fecal antigen assay, a urea breath test, a rapid urease test, and histology of a biopsy specimen. The real-time PCR assay for H. pylori is a specific and efficient method for detecting culture isolates. It provides a rapid and reliable detection of H. pylori. The qPCR assay can detect the presence of a particular genome sequence of H. pylori and can be used as a complementary rapid high throughput screening method.

Key Features:

• The H. pylori qPCR assay kit contains mixtures of forward and reverse primers along with a TaqMan probe, which targets specific sequences.
• Optimized qPCR protocol: just add the master mix, primers-probe mix, and DNA template

16S Real time PCR Assay—Detection of bacterial species

A real-time PCR assay has been designed to detect a wide range of bacterial species utilizing 16S rRNA sequences. The assay targets multiple bacterial species using conserved 16S rRNA sequences. The assay uses an oligonucleotide probe labeled with a fluorophore and quencher attached to the 5'-end (reporter) and 3'-end, respectively. The 16S TaqMan assay allows the detection and quantification of multiple bacterial species.

Potential Research Use (RUO) Applications:

Test for bacterial sepsis: Rapid and reliable detection of culture-positive neonatal sepsis, urine, or bloodstream infection for bacterial detection.
Cat# J16S-FAM
96 reactions (20 microlite reaction set up)

Key Features:

• The qPCR kit has forward and reverse 16S-specific primers that target the partial V6 region.
• These primers have universal M13 forward or reverse sequences at 5' ends.
• Using universal primers (M13 forward and reverse).
• Sanger cycle sequencing analysis of 16S amplified sequences can help identify bacterial isolates.

Nucleic Acid Extraction Buffer kit for DNA/RNA (XTRACK)

The extraction of high-quality nucleic acid from a given sample is the first and most crucial step in downstream DNA or RNA analysis in molecular biology. The XTRACK nucleic acid extraction buffer comprises non-toxic reagents that can lyse cells and release nucleic acids. This extraction buffer offers a simple lysis approach to release nucleic acids in the buffer. Following this, contaminant removal can be performed through a straightforward silica column-based washing process using commonly available 70% isopropyl alcohol. Finally, the nucleic acids can be quickly recovered with TE buffer (10mM TRIS pH8.0 and 0.1mM EDTA) or nuclease free water.

Potential Research Use (RUO) Applications:

The supplied nucleic acid extraction buffer is 2X concentrated. Any commercially available silica column can process volumes as low as 200 to 700 microliters (nucleic acid extraction buffer + sample).

Key Features:

• No Hazardous chemicals
• Cost-Effective
• Simultaneous extraction of high-quality DNA and RNA
• Easy to scale up sample volume

Cat# XTRACK50
Kit size: 50 samples preparation kit