Helicobacter pylori is a gram-negative bacterium formerly known as Campylobacter pylori. The bacterium is helical-shaped and flagellated. Bacterial infection can cause peptic ulcers in 10 to 20% of people, lymphoma of the gastric mucosa-associated lymphoid tissue, and a risk of 1 to 2% acquiring gastric adenocarcinoma stomach cancer. H. pylori spreads through direct contact with saliva, vomit, or stool from person to person. Contaminated water can also transmit H. pylori, particularly in areas with poor sanitation and clean water.
H. pylori is diagnosed through various methods, including a fecal antigen assay, a urea breath test, a rapid urease test, and histology of a biopsy specimen. The real-time PCR assay for H. pylori is a specific and efficient method for detecting culture isolates. It provides a rapid and reliable detection of H. pylori. The qPCR assay can detect the presence of a particular genome sequence of H. pylori and can be used as a complementary rapid high throughput screening method.
The
H. pylori qPCR assay kit contains mixtures of forward and
reverse primers along with a TaqMan probe, which targets
specific sequences.
Optimized qPCR protocol: just add the master mix,
primers-probe mix, and DNA template| Product Name | Package Size | Catalogue Number |
|---|---|---|
| Helicobacter pylori Real time PCR Assay | 96 reactions (20 microliter reaction set up) | JHP-FAM-1100 |
The extraction of high-quality nucleic acid from a given sample is the first and most crucial step in downstream DNA or RNA analysis in molecular biology. The XTRACK nucleic acid extraction buffer comprises non-toxic reagents that can lyse cells and release nucleic acids. This extraction buffer offers a simple lysis approach to release nucleic acids in the buffer. Following this, contaminant removal can be performed through a straightforward silica column-based washing process using commonly available 70% isopropyl alcohol. Finally, the nucleic acids can be quickly recovered with TE buffer (10mM TRIS pH8.0 and 0.1mM EDTA) or nuclease free water.
The supplied nucleic acid extraction buffer is 2X concentrated. Any commercially available silica column can process volumes as low as 200 to 700 microliters (nucleic acid extraction buffer + sample).
No
Hazardous chemicals
Cost-Effective
Simultaneous extraction of high-quality DNA and RNA Easy
to scale up sample volume| Product Name | Package Size | Catalogue Number |
|---|---|---|
| Nucleic acid extraction buffer kit for DNA/RNA | 50 samples preparation kit | XTRACK50 |